Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease
Authors/Creators
- 1. California Institute of Technology
- 2. University of Southern California
- 3. Icahn School of Medicine at Mount Sinai
Description
Kidney injury disrupts the intricate renal architecture and triggers limited regeneration, and injury-invoked inflammation and fibrosis. Deciphering molecular pathways and cellular interactions driving these processes is challenging due to the complex renal architecture. Here, we apply single cell spatial transcriptomics to examine ischemia-reperfusion injury in the mouse kidney. Spatial transcriptomics reveals injury-specific and spatially-dependent gene expression patterns in distinct cellular microenvironments within the kidney and predicts Clcf1-Crfl1 in a molecular interplay between persistently injured proximal tubule cells and neighboring fibroblasts. Immune cell types play a critical role in organ repair. Spatial analysis reveals cellular microenvironments resembling early tertiary lymphoid structures and identifies associated molecular pathways. Collectively, this study supports a focus on molecular interactions in cellular microenvironments to enhance understanding of injury, repair and disease.
Notes
Funding provided by: National Institutes of Health
ROR ID: https://ror.org/01cwqze88
Award Number: NIDDK UC2DK126024
Funding provided by: Deutsche Forschungsgemeinschaft
ROR ID: https://ror.org/018mejw64
Award Number: GE 3179/1-1
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Additional details
Related works
- Is cited by
- 10.1101/2023.11.22.568217 (DOI)