Published June 4, 2018 | Version v1
Dataset Open

The process of HDAC11 Assay Development: linearity and Km calculation

Creators

  • 1. Structural Genomics Consortium/University of Toronto

Description

With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.

 

Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).

          2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.

Notes

The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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4june2018-HDAC11 assay deve process-linearity-Km.pdf

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