Underlying and Extended Data for Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
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Truszkowski, Lukasz1
- Bottini, Sveva1
- Bianchi, Sara1
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Scrivano, Mirko G.1
- Ferrari Ramondo, Giulia2
- Belluci, Linda1
- Bell, Helen3
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Becca, Silvia1
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Snijders, Kirsten E.1
- Savorè, Giulia1
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Sozza, Federica1
- Ricca, Irene1
- Rubinetto, Cristina1
- Ferrera, Luana3
- Neri, Francesco1
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Ditadi, Andrea2
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Oliviero, Salvatore4
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Balmas, Elisa1
- Elton, Catherine3
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Bertero, Alessandro1
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1.
University of Turin
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2.
IRCCS Ospedale San Raffaele
- 3. Qkine
- 4. University of Torino
Description
Extended Data and raw data for the manuscript "Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering"
Extended Data 1.zip - protocol for preparation of the supplement for hE8 and B8+ media
Extended Data 2.zip - Gene counts, supporting files, and output results of bulk analyses
Extended Data 3.zip - Images of iPS cells adapted to cE8, hE8 and B8+ taken 24, 48 and 72 hours after passage. Contains raw .tiff files for each image and a .pdf with a compiled figure
Extended Data 4.zip - Results of miloR analysis on the differences in the distribution of cells adapted to cE8, hE8 and B8+ to assigned monocle clusters
Manuscript Data.zip – Raw data underlying the Figures 1, 2, 4, 5, 6 and 7.
Bulk_RNA_seq_archive – archived source code used for generating results in Figure 3
Files
Bulk_RNA_seq_archive.zip
Files
(5.8 GB)
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Additional details
Related works
- Is published in
- Publication: 10.12688/openreseurope.18245.1 (DOI)
Funding
References
- Truszkowski L, Bottini S, Bianchi S et al. Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering [version 1; peer review: awaiting peer review]. Open Res Europe 2024, 4:192 (https://doi.org/10.12688/openreseurope.18245.1)