Published May 15, 2018
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Use of antibiotics to optimize micropropagation of Betula pendula var. carelica Merckl.
Description
Dependence of the regenerative capacity of single-bud segments of Betula pendula Roth. var. carelica Merckl. shoots on the presence of some antibiotics in a nutrient medium are discussed in the paper.
Methods: cell culture in vitro; statistical analysis.
Β–lactam antibiotics are optimal at the stage of multiplication of Karelian birch in vitro with use of single–bud shoot segments as explants. Addition of carbenicillin (500 mg/l) and/or cefotaxime (500 mg/l) to the growth medium allows supporting visually clean tissue culture with no significant negative impact on morphometric parameters and viability of micro–plants.
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References
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