DEVELOPMENT OF CELL CULTURE SYSTEM FROM SELECTED TISSUES OF PANGAIUS HYPOPTHALMUS

Cell line provides a valuable biological tool for carrying out investigations into physiology, virology, toxicology, carcinogenesis, and transgenesis. The operation by which cells are grown under the control conditions, generally outside their natural habitat, is called as cell culture. Cells are isolated from the animals and plants cells, these isolated cells are allowed to grow and culture in artificial media. The cell lines from different tissues of different species are valuable for studying species-specific responses to viral infection at the cellular level. Cell culture systems were developed from different tissues of P.Hypopthalmus Namely Caudal Fin, heart, swim bladder, gills and eye tissues. A total of seven explants were prepared, but only two were used to develop cell culture. Cell culture systems developed from fin and heart were successfully subcultured to establish cell lines designated as PHCF respectively. Morphological evaluation under the inverted microscope revealed that all the explants prepared from fin explants were found to be adequately attached after 18-24 hours of explant preparation. The fish cell line is dominant in vitro tool for studying epidemiology, molecular carcinogenesis, toxicology and functional genomics in fish.