Published April 30, 2018 | Version v1
Dataset Open

The process of HDAC11 Assay Development: enzyme stability

Creators

  • 1. Structural Genomics Consortium/University of Toronto

Description

Before performing the kinetic study for calculating the Km for HDAC11, it is important to know the duration of the stability of the protein under the assay conditions. This is being analyzed here.

 

Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).

          2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.

Notes

The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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30april2018-HDAC11 assay deve process-enzyme stability.pdf

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