Journal article Open Access

Structural organization of the 5′ flanking region of the human corticotropin releasing hormone gene

Vamvakopoulos, Nicholas C.; Chrousos, George P.

We have determined the nucleotide sequence of the proximal 3625 nucleotides 5′ flanking the major mRNA start site of the human corticotropin releasing hormone gene (hCRH) and identified several putative regulatory elements. Interestingly, we did not detect any glucocorticoid responsive elements; we did however find five interspersed perfect half palindromic estrogen responsive elements, which might confer estrogen regulatability to the hCRH gene. We have identified a segment spanning from-2835 to-2972, which has about 72% homology to the 3′ terminal half of the human Alu I family of highly repetitive elements, and another one, which spans from-2213 to-2580 and has greater than 80% homology to members of human type O family of repetitive elements. These elements may confer DNA fragility, since the foci for hCRH and the human fragile site FRA8F colocalize in human chromosome 8. The structural information reported represents a first step in the study of regulation of the hCRH gene at the molecular level.

Files (811.8 kB)
Name Size
article.pdf
md5:54ab3465ac699d2b0335288d29a69962
811.8 kB Download
62
43
views
downloads
Views 62
Downloads 43
Data volume 34.9 MB
Unique views 62
Unique downloads 43

Share

Cite as