Journal article Open Access

Specificity of blebbistatin, an inhibitor of myosin II

Limouze, John; Straight, Aaron F.; Mitchison, Timothy; Sellers, James R.

Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. We have examined the specificity and potency of the drug by assaying its effects on the actin-activated MgATPase assay of diverse members of the myosin superfamily. Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 microM. Interestingly, smooth muscle which is highly homologous to vertebrate nonmuscle myosin is only poorly inhibited (IC50=80 microM). The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X.

Files (188.4 kB)
Name Size
article.pdf
md5:b485805c64975252e7dc4226e5228133
188.4 kB Download
98
91
views
downloads
Views 98
Downloads 91
Data volume 17.1 MB
Unique views 97
Unique downloads 89

Share

Cite as