Journal article Open Access

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System

Shen, Zhiyuan; Pardington-Purtymun, Paige E.; Comeaux, Jarmon C.; Moyzis, Robert K.; Chen, David J.

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.
Files (315.7 kB)
Name Size
article.pdf
md5:a0535b4bc76fb1456ab56783cb38cf67
315.7 kB Download
204
385
views
downloads
Views 204
Downloads 385
Data volume 121.6 MB
Unique views 191
Unique downloads 371

Share

Cite as