Published April 16, 2018 | Version v1
Dataset Open

SILVA_123 Eukaryota taxonomic training data formatted for DADA2; plus version with (uncurrated) additional sequences

  • 1. Leibniz Institute for Zoo and Wildlife research

Description

This is the Silva123 taxonomy reformatted to work with eukaryote sequences for dada2:

SILVA_123_dada2.fasta

It has been created using a script available here.

To increase coverage (Silva has a low coverage for eukaryotes). Uncurrated additional sequences similar to ASVs found in the intestine of hyenas (BLAST) have been added. The script used for taxonomic annotation of these sequences is available here. The file containing these additional (uncurrated!) sequences is:

SILVA_123_dada2_exp.fasta

This expanded file has been used for taxonomic annotation in: 

Heitlinger, E., Ferreira, S., Thierer, D., Hofer, H., & East, M. L. (2017). The intestinal eukaryotic and bacterial biome of spotted hyenas: the impact of social status and age on diversity and composition. Frontiers in cellular and infection microbiology, 7, 262.

Notes

This is the version used in: Emanuel Heitlinger, Susana C. M. Ferreira, Dagmar Thierer, Heribert Hofer, and Marion L. East (2017) The Intestinal Eukaryotic and Bacterial Biome of Spotted Hyenas: The Impact of Social Status and Age on Diversity and Composition Front. Cell. Infect. Microbio; 7: 262. doi: 10.3389/fcimb.2017.00262

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