Published April 10, 2018 | Version v1
Journal article Open

Effect of Growing Liver Biological Set on Various Cellular Cultures

Creators

  • 1. Sechenov First Moscow State Medical University
  • 2. Lomonosov Moscow State University
  • 3. Harbin Medical University

Description

Aim. To analyze the effect of growing liver biological set on viability and proliferative activity of various cellular cultures in vitro.

Material and Methods. The biological combination is an extract from the growing liver obtained by using of original technique. We  have assessed extract’s effect on the following cellular lines: hepatic  carcinoma Huh7, L-fibroblasts of mice, murine bone marrow  mesenchymal stem cells. Viability and proliferative activity were  assessed by staining the cells with trypan blue and visual counting of cells under phase contrast microscopy.

Result. Biological combination from neonatal piglet’s growing liver dose-dependently protects hepatocyte-like cells from deprivation of  fetal serum and stimulates cellular growth in presence of serum.  High concentrations of HRS do not lead to growth arrest of the Huh7  cells. At the same time, it is a cytostatic (or cytotoxic) for murine L- fibroblasts. Limited protective effect of the combination on the  deprivation of serum when exposed to bone marrow stem cells was revealed.

Conclusion. Our data show that the extract may be considered as an important regulator of reparative regeneration of liver with  protective and/or stimulating effect on mesenchymal stem and  hepatic-like cells and cytostatic effect on fibroblasts. So, further trials are necessary.

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