Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification
Creators
- 1. Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway
- 2. National Institute of Aquatic Resources, Section of Oceanography and Climate, Technical University of Denmark, Charlottenlund, Denmark
- 3. Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, UK
- 4. Sir Alister Hardy Foundation for Ocean Sciences, Plymouth, UK
- 5. Department of Bioscience, Aarhus University, Roskilde, Denmark
- 6. Department of Marine Sciences, University of Connecticut, Groton, CT, USA
Description
Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae.
Files
Smolina_Mol_Ecol_Resour.pdf
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