BACTERIOLOGICAL AND MOLECULAR STUDIES ON BACTERIATRANSMITTED FROM FISHES TO HUMAN.

A total of 1500 samples from 300 apparently healthy fish (280 Tilapia nilotica and 20 Catfish) were collected randomly from some markets and farms at Kafr El-sheikh governorate, Egypt. Also 50 human skin swabs were collected. These samples were collected to isolate and characterize bacteria transmitted from fish to human. In this study the prevalence of Staphylococcus aureus, Escherichia coli, Salmonella spp., Pseudomonas spp., Aeromonas spp. and Edwardseillatarda in Tilapia were 8.2, 21.4, 19, 11.4, 10 and 1.1% respectively, the prevalence of theses pathogens in Catfish were 5, 75, 60, 15, 10 and 0% respectively while its prevalence in human were 8, 24, 20, 12, 0 and 0% respectively. Escherichia coli serotype isolates from fish were O153, O1, O125 and O78. Salmonella serotype isolates from fish were Salmonellainganda, Salmonellatyphimurium and Salmonella Kentucky and Salmonellamolade. Results showed that all the eight isolates of Staphylococcusaureus subjected for determination of clfA gene were positive and negative to Hla gene, all the five Salmonella isolates were positive to invA and sefA genes and only one from four isolates of Escherichia coli was positive to eaeA gene and the four isolates were negative to Hly gene.