FIGURE 3. Chromosome I in Taxonomic status of the black fly Prosimulium italicum Rivosecchi (Diptera: Simuliidae) based on genetic evidence

FIGURE 3. Chromosome I of Prosimulium italicum. A. Proximal sections 22–27 of IL of female larva (Italy, Campania), showing nucleolar organizer (N.O.), presence of heteroband IL 25hb (arrow) in heterozygous configuration. The distal breakpoint (arrow) is also shown for perinucleolar inversion IL-15 of P. hirtipes from Slovakia (Morava). B. IS (except sections 1–5) and base of IL with nucleolar organizer (N.O.) of male larva (Italy, Sicily), showing IS-8 sequence and Y-linked IS-25 (heterozygous) with heteroband IS 10hb (+). Breakpoints are indicated for X-linked IS-9 and IS-27 in P. italicum. Breakpoints are also indicated for X-linked IS-10, autosomal polymorphism IS-26, and IL-15 (proximal breakpoint, arrow) in P. hirtipes; IS-9 is fixed in P. hirtipes. C = centromere. C. End of IS of female larva (Italy, Campania), showing distal breakpoints of X- linked IS-9 and IS-10.