Effect of Turmeric (Curcuma longa) Black Pepper (Piper nigrum) and Azolla Pinnata on Waste Water Nile tilapia Fish

The objective of this study was to investigate the effect of Turmeric (Curcuma longa) with Black Pepper (Piper nigrum) and Azolla Pinnata on Nile tilapia fish weight and the gene expression of growth hormone receptor (GHR) and insulin as growth factor (IGF) of Nile tilapia collected from waste water.  A total of 250 Nile tilapia fish divided into 5 groups each of 50. The first group of apparently healthy fish collected from Central Laboratory for Aquaculture Research El-Abbassa (clean water). The second group collected from waste water of Bahr El-Baqar zone. The third group collected from waste water of Bahr El-Baqar zone and fed on 0.5% (5g/kg diet) of each Turmeric (Curcuma longa) and Piper nigrum for 45 days. The fourth group collected from waste water of Bahr El-Baqar zone in the presence of small amount of Azolla Pinnata (Aquatic fern) for 45 days (Azolla grows quickly without feeding on Turmeric and Piper nigrum), while the fifth group collected from waste water of Bahr El-Baqar zone and fed on Turmeric (Curcuma longa) and Piper nigrum for 45 days in presence of Azolla Pinnata. The results cleared that the weight of fish in the first group showed an increase after 45 days but a slight decrease in weight were noticed in the second group in the first 15 days. There was an increase in fish weights in third and fourth groups and a marked increase in the fifth group after 45 days. Moreover, an increase in both GHR and IGF in groups fed on Turmeric and Piper nigrum for 45 days as well as in the presence of Azolla Pinnata.