Ficus deltoidea Extract Protects HaCaT Keratinocytes from UVB Irradiation-Induced Inflammation
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Description
Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.
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References
- K. B. Beckman, and B. N. Ames, "The free radical theory of aging matures,". Physiol. Rev., vol. 78, pp. 547–581, 1998.
- S. Pillai, C. Oresajo, and J. Hayward, "Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation — a review,". Int. J. Cosmet. Sci., vol. 27, pp. 17–34, 2005.
- A. Pupe, R. Moison, P. De Haes, G. B. Van Henegouwen, L. Rhodes, H. Degreef, and M. Garmyn, "Eicosapentaenoic acid, a n-3 polyunsaturated fatty acid differentially modulates TNF-alpha, IL-1alpha, IL-6 and PGE2 expression in UVB irradiated human keratinocytes,". J. Invest. Dermatol., vol. 118, pp. 692–698, 2002.
- C. E. M. Griffiths, G. Russman, G. Majmudar, R. S. Singer, T. A. Hamilton, and J. J. Voorhees, "Restoration of collagen formation in photodamaged human skin by tretinoin (retinoic acid),". N. Engl. J. Med., vol. 329, pp. 530-534, 1993.
- L. J. McCawley, and L. M. Matrisian, "Matrix metalloproteinases: they're not just for matrix anymore,". Curr. Opin. Cell. Biol., vol. 13, pp. 534–540, 2001.
- E. J. Jung, J. Lee, J. Baek, K. Jung, S. Huh, S. Kim, J. Koh, and D. Park, "Effect of Camellia japonica oil on human type I procollagen production and skin barrier function,". J. Ethnopharmacol., vol. 112, pp. 127–131, 2007.
- S. R. Pinnell, "Cutaneous photodamage, oxidative stress, and topical antioxidant protection". J. Am. Acad. Dermatol., vol. 48, pp. 1–19, 2003.
- I. A. Jahan, N. Nahar, M. Mosihuzzaman, B. Rokeya, L. Ali, A. K. Azad Khan, T. Makhmur, and M. Iqbal Choudhary, "Hypoglycaemic and antioxidant activities of Ficus racemosa Linn. Fruits,". Nat. Prod. Res., vol. 23, pp. 399-408, 2009.
- R. K. Singh, S. Mehta, D. Jaiswal, P. K. Rai, and G. Watal, "Antidiabetic effect of Ficus bengalensis aerial roots in experimental animals,". J. Ethnopharmacol., vol. 123, pp. 110-114, 2009. [10] A. Grone, "Keratinocytes and cytokines,". Vet. Immunol. Immunopathol., vol. 88, pp. 1–12, 2002. [11] L. Rittie, and G. J. Fisher, "UV-light-induced signal cascades and skin aging,". Ageing Res. Rev., vol. 1, pp. 705–720, 2002. [12] Z. Adam, M. Hamid, A. Ismail, and S. Khamis, "Effect of Ficus deltoidea extract on hepatic basal and insulin-stimulated glucose uptake,". J. Biol. Sci., vol. 9, pp. 796-803, 2009. [13] M. R. Sulaiman, M. K. Hussain, Z. A. Zakaria, M. N. Somchit, S. Moin, A. S. Mohamad, and D. A. Israf, "Evaluation of the antinociceptive activity of Ficus deltoidea aqueous extract,". Fitoterapia, vol. 79, pp. 557-561, 2008. [14] M. J. Oh, M. Abdul Hamid, S. Ngadiran, Y. K. Seo, M. R. Sarmidi, and C. S. Park, "Ficus deltoidea (Mas cotek) extract exerted antimelanogenic activity by preventing tyrosinase activity in vitro and by suppressing tyrosinase gene expression in B16F1 melanoma cells,". Arch. Dermatol. Res., vol. 303, pp. 161-170, 2010. [15] Z. Abdullah, H. Khalid, I. Zhari, and M. A. Rasadah, "Antiinflammatory activity of standardized extracts of leaves of three varieties of Ficus deltoidea,". Int. J. Pharm. Clin. Res., vol. 1, pp. 100-105, 2009.