Study of Optical Properties of a Glutathione Capped Gold Nanoparticles Using Linker (MHDA) by Fourier Transform Infra Red Spectroscopy and Surface Enhanced Raman Scattering
and E. Sheregii
16-Mercaptohexadecanoic acid (MHDA) and tripeptide glutathione conjugated with gold nanoparticles (Au-NPs) are characterized by Fourier Transform InfaRared (FTIR) spectroscopy combined with Surface-enhanced Raman scattering (SERS) spectroscopy. Surface Plasmon Resonance (SPR) technique based on FTIR spectroscopy has become an important tool in biophysics, which is perspective for the study of organic compounds. FTIR-spectra of MHDA shows the line at 2500 cm-1 attributed to thiol group which is modified by presence of Au-NPs, suggesting the formation of bond between thiol group and gold. We also can observe the peaks originate from characteristic chemical group. A Raman spectrum of the same sample is also promising. Our preliminary experiments confirm that SERS-effect takes place for MHDA connected with Au-NPs and enable us to detected small number (less than 106 cm-2) of MHDA molecules. Combination of spectroscopy methods: FTIR and SERS – enable to study optical properties of Au- NPs and immobilized bio-molecules in context of a bio-nano-sensors.
A.B. Descalzo, R. Mart├¡nez-M├í├▒ez, F. Sancen├│n, K. Hoffmann, K.
Rurack, "Hybrid Nanoapatite by Polysaccharide Nanogel-templated
Mineralization," Angewandte Chemie International Edition, Vol.45,
2006, pp. 5924-5948 2006.
I. H. El-Sayed, X.Huang, M.A. El-Sayed, "Surface plasmon resonance
scattering and absorption of anti-EGFR antibody conjugated gold
nanoparticles in cancer diagnostics: applications in oral cancer," Nano
Letters. Vol. 5, 2005, pp. 829-834.
J. Kimling, M. Maier, B. Okenve, V. Kotaidis, H. Ballot, A. Plech,
"Turkevich Method for Gold Nanoparticle Synthesis Revisited," Journal
Physical Chemistry B, Vol. 110, 2006, pp. 15700-15707.
Liu, D.Leech, H. Ju, "Application of Colloidal Gold in Protein
Immobilization, Electron Transfer, and Biosensing," Analytical Letters,
Vol. 36, , 2003, pp. 1-19.
M. Hu, J. Chen, ZY. Li, L. Au, G.V. Hartland, X. Li, M. Marquez, Y.
Xia, "Gold nanostructures: engineering their plasmonic properties for
biomedical applications, "Chemical Society Reviews, Vol. 35, 2006,
N. Stasyuk, R. Serkiz, S. Mudry, G. Gayda, A. Zakalskiy, Y.
Koval-chuk, M. Gonchar, M. Nisnevitch, "Recombinant human arginase
I immobilized on gold and silver nanoparticles: preparation and
properties," Nanotechnology Development, Vol.1, 2011, p. 11-15. S.
 V. Leff, L. Brandt, J. R. Heath, "Synthesis and Characterization of
Hydrophobic, Organically-Soluble Gold Nanocrystals Functionalized
with Primary Amines," Langmuir, Vol. 12, 1996, pp. 4723-4730.
 H.Shayani-Jam,D.Nematollahi,"Electrochemical evidences in oxidation
of acetaminophen in the presence of glutathione and N-acetylcysteine,"
Chemical Communications, Vol. 46, 2009, pp. 409-411.
 M. Hasan, D. Bethell, M. Brust, "The fate of sulfur-bound hydrogen on
formation of self-assembled thiol monolayers on gold: (1)H NMR
spectroscopic evidence from solutions of gold clusters," Journal of the
American Chemical Society, Vol. 124, 2002, pp. 1132-1133.
 M. J. Hostetler, J.J. Stokes, R.W. Murray, "Infrared Spectroscopy of
Three-Dimensional Self-Assembled Monolayers: N-Alkanethiolate
Monolayers on Gold Cluster Compounds," Langmuir, Vol. 12, 1996, pp.
 Y. Sahoo, H. Pizem, T. Fried, D. Golodnitsky, L. Burstein, C.N.
Sukenik, G. Markovich, "Alkyl Phosphonate/Phosphate Coating on
Magnetite Nanoparticles: A Comparison with Fatty Acids," Langmuir,
Vol. 17, 2001, pp.7907-7911.
R. Aroca, Surface Enhanced Vibrational Spectroscopy, John Wiley &
Sons Ltd., Chichester, 2006.
R.J. Silbey, R.A. Alberty, and M. G. Bawendi, Physical Chemistry, John
Wiley & Sons Ltd., New York, 2004.
S.J. Guo, E.K. Wang, "Synthesis and electrochemical applications of
gold nanoparticles," Analytica Chimica Acta, Vol. 598, 2007, pp. 181-