VOLTAMMETRIC BEHAVIOR AND DETERMINATION OF VINCAMINE IN PURE FORM, PHARMACEUTICAL FORM AND BIOLOGICAL FLUIDS

Voltammetric oxidation of Vincamine was studied in Britton - Robinson buffer by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square-wave voltammetry (SWV) at glassy carbon electrode (GCE). These sensitive validation and consistent reproducible voltammetric technique were investigated for the trace quantification of the drug in pure, human urine and in pharmaceutical formulations. The optimum experiential parameters were by way of follow: Britton - Robinson (BR) (pH 6.0). The peak current exhibited a linear correlation with the drug concentration in the range limit of 0.658 - 8.36μg/ml,1.65-24.75 μg/ml, with the limit of detection 0.502, 1.67μg/ml and limit of quantitation were 0.066, 0.382μg/ml Differential pulse and Square Wave Voltammetry respectively. The proposed voltammetric procedures successfully applied for determination of VIN in its pure form and pharmaceutical formulation using GCE. The method allows a simple, rapid and reproducible determination of this compound without any interference from other ingredients present and furthermore applicability was tested in urine samples.