Concentrations of dissolved dimethyl sulfide (DMS), methanethiol and other trace gases in context of microbial communities from the temperate Atlantic to the Arctic Ocean
Creators
- 1. Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research
- 2. Laboratoire des Sciences du Climat et de l'Environnement, CNRS-CEA-UVSQ
- 3. Norwegian Polar Institute, Fram Centre
Description
This archive contains bioinformatic code and data files to reproduce bacterial analyses from Gros et al. (Biogeosciences; https://doi.org/10.5194/bg-2022-150). In this study, seawater samples collected along an Atlantic-Arctic transect (55-80°N) were evaluated for microbial community composition and trace gas concentrations. Microbes were sampled underway using "AUTOFIM" automatic filtration; and bacterial communities amplicon-sequenced using 16S rRNA gene primers. Raw sequence data is available at ENA under BioProject PRJEB50492.
The archive contains:
- Rmarkdown for primer-clipping, ASV generation and taxonomy assignment: 1_DADA.Rmd
- Rscript for ASV and metadata processing; alpha-diversity calculations: 2_DataLoad.R
- Rscript for generation of manuscript figures: 3_Results.R
- Rscript for generation of supplementary figures: 4_Supplement.R
- Metadata and environmental parameters as .txt files
Files
Appendix_Acetone.txt
Files
(1.7 MB)
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