Fig. 1 in DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals

Fig. 1. (A) Geographic distributions of the three northern European ringed seal subspecies from which cestodes were collected for COI barcoding: Baltic ringed seal (green), Saimaa ringed seal (blue), and Ladoga ringed seal (red). (B) Midpoint-rooted neighbor-joining tree based on K2P distances among COI barcode sequences of 35 cestode individuals collected from the three focal ringed seal subspecies. Individuals are colored according to host subspecies, numbers above or next to branches are bootstrap support values based on 500 resamplings of the data matrix (only values> 70% shown). Cestode species names indicated under the main branches are based on barcode similarity to reference sequences in GenBank. (C) Maximum-likelihood tree based on a 562-bp alignment of the barcode sequences of the focal cestodes and 34 diphyllobothriidean reference taxa obtained from GenBank. Numbers above branches are bootstrap support values based on 100 resamplings of the data (only values> 70% shown). In both trees, individual names include the voucher code or GenBank accession number, seal subspecies abbreviation with seal individual code, barcode-based cestode species name, and name of the host (sub)species from which the cestode specimen was collected. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)