Published July 29, 2024
| Version v1
Dataset
Open
HER2/neu-specific T-cells
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1.
Research Institute of Fundamental and Clinical Immunology
Description
Single-cell RNA-seq data of HER2/neu-specific T-cells genereated with the BD Rhapsody™ system.
Donor information: 3 healthy subjects, 2 male (SampleTag04, SampleTag05), 1 female (SampleTag06).
Cell preparation: HER2/neu-specific T-cells were obtained via a Tetramer-based sorting of the naturally-occuring anti-CD3 and anti-CD28-activated T-cells co-cultured with the Dendritic cells that were maturated by a KIFGSLAFL peptide.
Single-cell analysis system: BD Rhapsody™
Library strategy: 5' mRNA sequencing
Library preparation protocol: BD Rhapsody™ TCR/BCR Full Length, Targeted mRNA, and Sample Tag Library Preparation
mRNA panel: BD Rhapsody™ Immune Response Panel HS
BD Pipeline version: 1.11.1L