Towards the Standardization of the Dark/Light Transition Test with Danio rerio embryos - Preliminary Results from an Inter-laboratory Ring Test
Creators
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Massei, Riccardo
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Christou, Maria1
- Schroeder, Katja2
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Ottermanns, Richard
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Domingues, Inês3
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Pavlaki, Maria3
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Monteiro, Marta3
- Zwart, Edwin4
- Hessel, Ellen4
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Johann, Sarah5
- Knapen, Dries6
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Vergauwen, Lucia6
- Huyghe, Kato6
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Kjær, Pernille Ambus7
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Holbech, Henrik7
- Molina, Beatriz8
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Muriana, Arantza8
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Sebire, Marion9
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Lillicrap, Adam1
- 1. Norwegian Institute for Water Research
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2.
RWTH Aachen University
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3.
Centro de Estudos Ambientais e Marinhos
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4.
National Institute for Public Health and the Environment
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5.
Goethe University Frankfurt
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6.
University of Antwerp
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7.
University of Southern Denmark
- 8. BBD BioPhenix SLU (Biobide)
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9.
Centre for Environment, Fisheries and Aquaculture Science
Description
One of the most commonly utilized behavioral assays with zebrafish embryos is the dark/light transition test (DLTT). This test involves recording and quantifying the swimming activities of zebrafish eleutheroembryos under different light conditions. The DLTT lacks standardization, and guidelines have yet to be established. As a result, zebrafish laboratories employ in-house protocols to record the activity of zebrafish eleutheroembryos. Different statistical tests are employed to explain the results and analyze the output. Consequently, comparing the results across different studies becomes a challenging task and poses a potential obstacle to bring these tests to regulators. Prior to developing a universal and standard guideline, it is necessary to characterize and understand the general variation in the DLTT.
Files
Poster_SETAC_LMR_Massei-MCH.pdf
Files
(2.4 MB)
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