Dataset #2 related to article "NaCl enhances CD8+ T-cell effector functions in cancer immunotherapy"
Creators
- Scirgolea, Caterina1
- Sottile, Rosa1
- De Luca, Marco1
- Carnevale, Silvia1
- Puccio, Simone2, 1
- Susanna, Alberto1
- Ferrari, Valentina1
- Scarpa, Alice1
- Scamardella, Eloise1
- Feno, Simona1
- Camisaschi, Chiara1
- De Simone, Gabriele
- Basso, Gianluca1
- Giuliano, Desiree1
- Mazza, Emilia Maria Cristina1
- Gattinoni, Luca3
- Roychoudhuri, Rahul4
- Voulaz, Emanuele5
- Di Mitri, Diletta5, 1
- Simonelli, Matteo5, 1
- Losurdo, Agnese1
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Pozzi, Davide1, 5
- Tsui, Carlson6
- Kallies, Axel6
- Barberis, Elettra7
- Manfredi, Marcello7
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Rescigno, Maria1, 5
- Jaillon, Sebastien5, 1
- Lugli, Enrico1
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1.
IRCCS Humanitas Research Hospital
- 2. UoS Milan
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3.
Leibniz Institute for Immunotherapy
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4.
University of Cambridge
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5.
Humanitas University
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6.
University of Melbourne
- 7. University of Piemonte
Description
CD8+ T cells control tumors but inevitably become dysfunctional. Ionic metabolism is emerging as a regulator of CD8+ T cells in anti-tumor immunity. We show that sodium chloride (NaCl) counteracts T-cell dysfunction to promote cancer regression. NaCl supplementation during CD8+ T-cell culture induced potent effector differentiation, IFN-g production and cytotoxicity while maintaining gene networks responsible for stem-like plasticity. Accordingly, adoptive transfer of tumor-specific T cells resulted in superior anti-tumor immunity in a humanized model. In mice, high-salt diet reduced growth of experimental tumors in a CD8+ T cell-dependent manner, by inhibiting terminal differentiation, and by enhancing the effector potency of CD8+ T cells. Mechanistically, NaCl enhanced glutamine consumption that was critical for transcriptional, epigenetic and functional reprogramming. In humans, CD8+ T cells undergoing antigen recognition in tumors and predicting favorable response to checkpoint blockade immunotherapy resembled those induced by NaCl. Thus, NaCl metabolism is a major regulator of CD8+ T-cell effector function, with potential translation in cancer immunotherapy.
Files
Dataset_4_raw_count_matrix.txt
Files
(40.6 GB)
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md5:3980d71f0c48f37f83acf8192ac7594b
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287.4 MB | Download |
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md5:644b82535e5651d475425ccc44fb48e4
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290.0 MB | Download |
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md5:4e3364502aa3daea45a26833b5a3e4ff
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286.3 MB | Download |
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md5:9ca2970499a0ecb1141f9db9d99cefd5
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285.5 MB | Download |
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md5:5eb216ae098415d103f616a812886415
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288.0 MB | Download |
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md5:335f10279724989f74e596dcf132d159
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279.4 MB | Download |
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md5:5e4174f203d1a5b6cd9eeaf858b7ffec
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287.5 MB | Download |
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288.4 MB | Download |
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md5:49e0944c9606de9728e5c52f0810c299
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283.2 MB | Download |
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md5:9a8fad929a30727bba6f546000c831ed
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289.3 MB | Download |
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md5:79489861a2a4a4764e67108d4b54ae27
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282.3 MB | Download |
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md5:a6b72223e14f67dbc0ce876173d4fff0
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289.2 MB | Download |
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md5:59fb2eb5da155663530ecd13918d54f5
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288.6 MB | Download |
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md5:43cf2c22a8a3d48364a5d88661e94bb5
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286.9 MB | Download |
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md5:a1b03c39cdba3245f5017b278c12d08e
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289.3 MB | Download |
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md5:2dc26c571a35cae460f756c20322eecb
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288.6 MB | Download |
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md5:8e5644c1049add75063efc8474d8f445
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282.3 MB | Download |
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md5:5c2ff8aee41d24091b80478bd880140e
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11.9 MB | Preview Download |
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md5:14c21ccc96b7df451bd3d92ee593c141
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2.9 GB | Download |
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md5:cae5172f09003a3973024ba37bc0295c
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2.8 GB | Download |
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md5:dde014e8d769630f8e34d12c1c95352d
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md5:5d607c33ab2aca777b44dcf2c5f350e2
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2.8 GB | Download |
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md5:c726023075b67757acc91c5402a838ee
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md5:23d8f9fa267d37aadb1cfd4c83a25025
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3.0 GB | Download |
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md5:391cdaa8c2b164888c6741c6393ea9e4
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2.8 GB | Download |
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md5:2d6afd0297fef377d6c14a6a0630d91b
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3.1 GB | Download |
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md5:ad34522040f5424b6ba27646ee3a7c86
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2.9 GB | Download |
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md5:8844dee59aaa38c2986194646fc04636
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3.3 GB | Download |
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md5:e68cfbc31e5fbaefcaa87551abb5365e
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2.5 GB | Download |
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md5:069d77542ec382c789a83ebf50244612
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2.8 GB | Download |
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md5:05d0d89057210007c90a56d59a339138
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24.9 kB | Download |