C2C12 and THP1 images Brightfield and Hoechst

20221207_1700_gt_C2C12 has 2x 96 TIF files with Brightfield and Hoechst stained images of C2C12 cells plated in 96 well plates the day before imaging

20231106_1300_mm_THP1 has 2x 96 TIF files with Brightfield and Hoechst stained images of THP1 cells cultured in 96 well plates for two days in 100ng/ml PMA, and 1 day in routine culture meidum before imaging.

Wells of the 96-well plates were imaged automatically using a Cytation-1 Cell imaging multimode reader (Agilent) set at 37 degrees Celsius using the Gen5 (version 3.12) software. For brightfield images, one image per well was captured with an Olympus 4x UPLFLN objective, using user-trained auto-focus and manual exposure settings that were set before imaging of each plate. For nuclei counting, 4 mM of Hoechst (33342, #B2261, Sigma) from a 40 mM stock solution was added 15 minutes before imaging to each well. Nuclei image fluorescence readings were taken using the same Olympus 4x UPLFLN objective and a 365 nm LED with an EX337/EM447 DAPI filter cube.