Annual dynamics and metagenomics of marine vesicles: a layer of complexity in the dissolved organic fraction

The data consists  second part of data for the same paper " Annual dynamics and metagenomics of marine vesicles: a layer of complexity in the dissolved organic fraction." Data were uploaded separately due to the limited space available for uploading.

The data are Illumina raw reads obtained from vesicle fractions of 25%, isolated from coastal Mediterranean seawaters in Alicante, Spain, over the course of one year. Illumina DNA prep kit (IDT, Ref. 20026930) was used following the manufacturer's protocol to generate and sequence the libraries for the obtained DNA samples from EVs fractions metagenomes. Subsequently, these libraries were sequenced using HiSeq X technology (150 PE, 1 lane) by two different facilities: Macrogen in Seoul, Republic of Korea, and the Genomics Unit of the Center for Genomic Regulation (CRG) in Barcelona, Spain. The dataset was used in order to ascertain the main microbial organisms involved in the production of EVs and packaging of DNA into those  EVs in our annual sampling  and the types of genes that were packaged in vessicles isolated from the Coastal Mediterranean seawaters (Functional annotation of genes packaged in vesicles).

 The data are labeled as  "EVs_25_DEC_1.fastq"  which denotes the vesicle 25% Optiprep fraction for sample  collected in December, with "1" indicating the forward read, while "EVs_25_DEC_2.fastq" represents the corresponding reverse read. This naming scheme is consistent across all vesicle fraction samples in the dataset.