Native MS dataset for: "Structural Insights into the Interaction Between Adenovirus 5C Hexon and Human Lactoferrin"

Native mass spectrometry dataset used in: Structural Insights into the Interaction Between Adenovirus 5C Hexon and Human Lactoferrin. Arun Dhillon, B. David Persson, Oleksandr Volkov, Hagen Sülzen, Alan Kádek, Petr Pompach, Sami Kereïche, Charlotte Uetrecht, Martin Lepšík, Niklas Arnberg and Sebastian Zoll. The Journal of Virology (2024). doi: 10.1128/jvi.01576-23

Description:

Native mass spectrometry (MS) analysis of the effect of solution ionic strength on the stoichiometry of interaction between human adenovirus HAdV-C5 hexon and human lactoferin.

Sample processing:

Prior to native MS measurements, hexon:Lf complex purified in buffer D was buffer exchanged into 20 mM aqueous ammonium acetate solution pH 7.0 (AA; ≥99.99% trace metals basis, Honeywell Research Chemicals) using two cycles of spin gel-filtration (MicroBioSpin P-6, Bio-Rad). The complex was subsequently diluted by 20 mM AA to 0.5 µM (estimated based on 3+3 stoichiometry). Alternatively, for low ionic strength conditions, the dilution was performed with LC-MS grade water instead, resulting in 5.85 mM AA concentration. Reference spectra of isolated Hexon and LF were obtained from 5 µM (monomer) samples in 150 mM AA pH 7.0.

After short equilibration on ice, the samples were introduced into an Orbitrap Q Exactive UHMR mass spectrometer (Thermo Scientific) via static nanoelectrospray ionization. The samples were electrosprayed from gold-coated borosilicate glass capillaries Kwik-Fil 1B120F-4 (World Precision Instruments) prepared in-house essentially as described before (PMID: 17406634, PMID: 33658206). The mass spectrometer was tuned for best signal quality and intensity, while maintaining minimal ion activation. Specifically, electrospray voltage was kept at 1.25 kV, source desolvation temperature 120°C, in-source trapping -50 V, ion transfer profile “high m/z”, analyzer profile “low m/z”, analyzer target resolution 1500 acquiring in mass range 1000 – 20000 m/z, averaging 5 microscans. Further desolvation and collisional cooling in HCD cell was performed with nitrogen at relative gas pressure setting 8.0 with gentle collisional activation by 10 V HCD voltage gradient.

Data processing:

Raw spectra were averaged over at least 50 scans in Thermo Xcalibur Qual Browser 4.2.47 (Thermo Scientific) and mass deconvoluted in UniDec 6.0.3 package (PMID: 25799115). The averaged spectra were exported for ZENODO deposition as single-scan Thermo .raw files (including instrumental parameters metadata) and in plain m/z vs intensity .txt files.