Dr. Manuele Gori

Supplementary Figure 1. Immunohistochemical analysis of the TJ and AJ proteins with unchanged expression.

LPS-treated vs. control monolayers did not show any differential expression or discontinuous distribution of tight junction protein 1 (TJP1, a), E-cadherin (CDH1, b) and b-catenin (CTNNB1, c) as well as in the ethanol-treated vs. control monolayers for TJP1 (d) as shown in the representative immunofluorescence micrographs. The far right histograms show the relative expression of the fluorescence intensity (FITC) on the cell surface of Caco-2 monolayers treated with LPS vs. controls (a-c) and ethanol vs. controls (d) by perimeter analysis. Data are reported as mean ± SEM; n = 3 independent experiments. Statistical analysis was performed using unpaired t-test. Scale bars: 25 μm. Legend: Ctrl (Control); LPS (Lipopolysaccharide); EtOH (Ethanol).