The ilha de Santa Catarina is situated in southern Brazil, Atlantic Forest, in the state of Santa Catarina, municipality of Florianópolis. Fish specimens were collected in 15 sampling points along the drainages of rio Córrego Grande, rio Palha, rio Ratones, rio Tavares, rio Vermelho, as well as in the channel of lagoa do Peri. Specimens belonging to 16 species (one of them exotic) were collected, distributed in 12 families and six orders.