Pristimantis uisae is a poorly known frog species reported only from three nearby localities in the western slopes of the Cordillera Oriental of Colombia (Boyacá and Santander Departments). In this work, 12 new records for this species are provided, including the first published records of this species for Cundinamarca Department and from the Eastern slopes of the Cordillera Oriental. Additionally, the new localities reported herein increase its known elevation range to 2300−3050 m. Further studies are needed for having a better understanding of the distribution of Pristimantis uisae.