Confocal microscopy images of Escherichia coli cells treated with various antibiotics (LB, exponential phase)

This dataset and CARE model is part of the publication "Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth".

It contains all CLSM images that were used for the publication, as well as the single-cell regions of interest for analyses.

Cells were grown to exponential phase in LB Lennox and antibiotics were added for 0-60 min. Cultures were then chemically fixed, immobilized and stained for DNA (DAPI) and membrane (Nile Red). The strain (NO34) expresses a MreBsw-sfGFP fusion protein from the native chromosomal locus. It was a kind gift from Zemer Gitai (Ouzounov et al., 2016).

More information can be found in the publication.