Published October 6, 2023 | Version v1
Dataset Open

MD simulation of a model Gram-positive bilayer membrane (60% PG, 35% lysyl-PG, 5% CL)

Authors/Creators

  • 1. University of South Florida

Description

Composition:

Model bilayer composition aimed at replicating the behavior of the Staphylococcus aureus membrane (See also Mohanan et al. Chem Sci 2020, 11, 4669: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159255/)

Per leaflet we have 48 POPG (16:0 / 18:1; -1 net charge), 28 DPPGK (16:0 / 16:0; +1 net charge), 4 TOCL1 (18:1,18:1 / 18:1,18:1; -1 net charge), giving 60% PG, 35% lysyl-PG, 5% CL. Bilayer is symmetric. 

Technical details:

OpenMM 7.5.1

CHARMM36m force field

6,712 TIP3P waters

150 mM KCl (64 K+, 16 Cl-)

Temperature = 303.15 K

Trajectory output every 100 ps

Trajectory length = 500 ns ( = 5,000 frames)

(Standard CHARMM-GUI relaxation protocol)

Files

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