Single cell RNASeq of virus-specific cellular immune responses in patients with chronic hepatitis B virus infection
Published October 2, 2023 | Version v1
Dataset Open

HBV Immunomics 10X data

Creators

  • 1. Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, Basel, Switzerland
  • 2. Service d'hépato-gastroentérologie, Pôle hépato-digestif, Institut Hospitalo-Universitaire de Strasbourg, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. / Institut de Recherche sur les Maladies Virales et Hépatiques, Inserm UMR_S1110, University of Strasbourg, Strasbourg, France
  • 3. ervice d'hépato-gastroentérologie, Pôle hépato-digestif, Institut Hospitalo-Universitaire de Strasbourg, Hôpitaux Universitaires de Strasbourg, Strasbourg, France
  • 4. Roche Pharma Research and Early Development, Immunology, Infectious Diseases and Ophthalmology (I2O) Discovery and Translational Area, Roche Innovation Center Basel, Basel, Switzerland
  • 5. Service d'hépato-gastroentérologie, Pôle hépato-digestif, Institut Hospitalo-Universitaire de Strasbourg, Hôpitaux Universitaires de Strasbourg, Strasbourg, France

Description

Here, we established a single-cell RNA-sequencing (scRNA-seq) dataset of HBV immune responses in patients to contribute to a better understanding of the dysregulated immune response in CHB. We collected  blood samples of a well-defined cohort of CHB and healthy controls including a subset of matched blood and liver samples. scRNA-seq data of 55,825 total immune cells as well as 131,367 sorted HBV-specific, non-naive and PD1+ T cells was generated using the 10X Genomics platform (186,123 cells) and the full-length Smart-seq2 protocol (1,069 cells). This unique scRNA-seq dataset serves as a resource describing T cell responses in patients and offers the opportunity to explore transcriptional changes underlying HBV-related immune cell dysfunction in blood and liver tissue and to identify new targets or biomarkers for HBV-related immune exhaustion.

This dataset incluces the 10X Genomics data only.

 

Files

standard_workflow_besca2.ipynb

Files (10.8 GB)

Name Size Download all
md5:caa0df813cae98974d457c7bf963803c
1.3 GB Download
md5:7da9cf0fcbb971e58e7874ddee02d229
4.3 GB Download
md5:0181697d13c78048057ba216ee068bd2
7.8 MB Download
md5:e2581aa9e390ce0c3f335952935b96d4
7.1 MB Preview Download
md5:7561bd452c1e6c26b923116f4ab88f1a
5.2 GB Download

Additional details

Related works

Is continued by
Dataset: 10.5281/zenodo.8399475 (DOI)
Dataset: 10.5281/zenodo.8399458 (DOI)