Published September 28, 2023
| Version 2
Dataset
Open
Malaria drives unique regulatory responses across multiple immune cells during human infection
Authors/Creators
- 1. QIMR berghofer
- 2. QIMR berhofer, University of Queensland
- 3. QIMR berghofer, QUT
- 4. Menzies school of health research
- 5. Infectious Diseases Unit, Queen Elizabeth Hospital,
- 6. Griffith University
Description
To investigate malaria driven transcriptional changes in specific immune cell subsets, we sorted live PBMCs from 6 malaria infected donors (day 0), and two subsequent time points after drug treatment (day 7 and 28), along with PBMCs from 2 healthy controls. We performed scRNAseq of these cells, and used clustering and sub-clustering to identify specific immune cell subsets. Differential gene analysis between day 0 and day 28 was performed for each cell cluster and sub-cluster. Key transcriptional changes were confirmed at the protein level with additional donor samples.
Files
Files
(4.1 GB)
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