Detection of salmon pathogen using a CRISPR/dCas9 based DNA assay on a photonic biosensor
Description
Aeromonas species are the most common source of bacterial infection in fish. High density of fish in aquaculture often leads to fast disease spread, massive fish death, and water contamination. Early detection of bacterial pathogens could provide better disease control.
Surfix Diagnostics has developed a photonic biosensor platform which can directly measure double stranded DNA (dsDNA) without requiring cell culture or DNA amplification. In PHOTO-SENS*, a demonstrator photonic biosensor system for salmon pathogen detection is being developed. The present study describes the detection of bacterial dsDNA using either PCR fragments or whole genomic DNA on Surfix’s photonic biosensor platform.
* This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 965643. See www.photo-sens.eu for more information.
Files
Detection of salmon pathogen using a CRISPR-dCas9 based DNA assay on a photonic biosensor (PHOTO-SENS_Surfix).pdf
Files
(298.0 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:4874f833f7ea677b47532c0da9f0eb78
|
298.0 kB | Preview Download |
Additional details
Funding
References
- Austin, B. Methods for the diagnosis of bacterial fish diseases. Mar Life Sci Technol, 1, 41–49 (2019). https://doi.org/10.1007/s42995-019-00002-5
- Besselink, G. et al. Asymmetric Mach Zehnder Interferometric Biosensing for Quantitative and Sensitive Multiplex Detection of Anti SARS CoV 2 Antibodies in Human Plasma. Biosensors 2022, 12 , 553. https://doi.org/10.3390/bios12080553
- Huang, T. et al. CRISPR Cas based techniques for pathogen detection: Retrospect, recent advances, and future perspectives J Adv Res 50 69 82 (2023) https://doi.org/10.1016/j.jare.2022.10.011