Gene editing using CRISPR/Cas9 to induce new mutations in the SYP121 gene in Arabidopsis and barley

We used the SYP121 gene as a target for CRISPR/Cas 9 based genome editing in both Arabidopsis and barley. We identified the barley SNARE with the FxRF motif in the first 15 residues of the protein in the target genotype Golden Promise. We then designed constructs that were expected to knock-out this part of the gene in Arabidopsis and in barley.