Published 2024 | Version v1
Dataset Open

Cilia motility and coverage analysis of hiPSC-derived airway epithelial cells

Creators

  • 1. Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Jeffrey Cheah Biomedical Centre, Puddicombe way, CB2 0AW, Cambridge, UK
  • 2. Department of Physics, University of Cambridge, Cavendish Laboratory, JJ Thomson Avenue, CB3 0HE, Cambridge, UK
  • 3. Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, Tennis Court Road, CB2 1QN, Cambridge, UK
  • 4. School of Physiology, Pharmacology and Neuroscience, Biomedical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK
  • 5. Center of Research and Development for Biomedical Instrumentation, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand
  • 6. Department of Clinical Neurosciences, Victor Phillip Dahdaleh Heart & Lung Research Institute, University of Cambridge, Papworth Road, Cambridge, CB2 0BB, UK
  • 7. Department of Medicine, Victor Phillip Dahdaleh Heart & Lung Research Institute, University of Cambridge, Papworth Road, Cambridge, CB2 0BB, UK
  • 8. UCL Great Ormond Street Institute of Child Health, University College London, WC1N 1EH, London, UK
  • 9. University of Cambridge, Molecular Immunity Unit, Department of Medicine, CB2 0AW, Cambridge, UK; University of Cambridge, Cambridge Centre for Lung Infection, Royal Papworth Hospital NHS Foundation Trust, CB2 0AY, Cambridge, UK
  • 10. Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, Tennis Court Road, CB2 1QN, Cambridge, UK; University of Cambridge, Department of Physiology, Development and Neuroscience, Tennis Court Road, CB2 1QN, Cambridge, UK
  • 11. Berlin Institute of Health at Charité, BIH Center for Regenerative Therapies, Augustenburger Platz 1, 10117, Berlin, DE; Max-Planck-Institute for Molecular Genetics, Ihnestraße 63-73, 14195, Berlin, DE; Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Jeffrey Cheah Biomedical Centre, Puddicombe way, CB2 0AW, Cambridge, UK

Description

Ciliary beat frequency and cilia coverage are key parameters of the maturation and function of airway epithelia. In our study "In vitro platform to model the function of ionocytes in the human airway epithelium" we have reported the differentiation of airway epithelia from human induced pluripotent stem cell (hiPSCs) with similar properties to those derived from primary cells. We have used this platform to study the effect of the KO of FOXI1 and, as a consequence, the depletion of ionocytes on the function of the airway epithelium. Cilia motility and coverage were among the assessed parameters.

This dataset includes ciliary beating frequency and cilia coverage data of hiPSC-AECs obtained from up to 20 high-speed microscopy videos per biological replicate using a Fourier transform method described in Fradique, Causa et al., 2023, Royal Society Open Science (https://doi.org/10.1098/rsos.230185). 

Files

FOXI1KO A KO hiPSC-AECs ALI Figure 5.csv

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