Implications of a multiscale structure of the yeast Nuclear Pore Complex
Authors/Creators
- 1. Department of Pharmacology, Physiology and Biophysics, Boston University
- 2. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco
- 3. Laboratory of Cellular and Structural Biology, The Rockefeller University
- 4. Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Description
Nuclear Pore Complexes (NPCs) direct the nucleocytoplasmic transport of
macromolecules. Here we provide a composite multiscale structure of the yeast NPC
based on improved 3D density maps from electron cryo-microscopy and AlphaFold2
atomic models. Key features of the inner and outer rings were integrated into a
comprehensive model. We resolved flexible connectors that tie together the core scaffold,
along with equatorial transmembrane complexes and a lumenal ring that anchor this
channel within the pore membrane. The organization of the nuclear double outer ring
reveals a common architecture that may be shared with ancestral NPCs. Additional
connections between the scaffold and the central transporter suggest that, under certain
conditions, a degree of local organization is present at the periphery of the transport
machinery. These connectors may couple conformational changes in the core scaffold to
the central transporter to modulate transport. Collectively, this analysis provides insights
into assembly, transport and NPC evolution.
Files
data.zip
Additional details
Software
- Repository URL
- https://github.com/integrativemodeling/NPC_TMDs