Deep and fast label-free Dynamic Organellar Mapping - Imaging data

Imaging data from the article "Deep and fast label-free Dynamic Organellar Mapping", published in Nature Communications by Schessner et al., from Fig. 6, 7 and Supp. Fig. 6c.

Widefield images were captured on a Leica DMi8 inverted microscope equipped with an iTK LMT200 motorised stage, a 63x/1.47 oil objective (HC PL APO 63x/1.47 OIL) and a Leica DFC9000 GTC Camera, and controlled with LAS X (Leica Application Software X).

Fig6_GLG1_TGOLN2_GALNT2_SuppFig6c_LC3B: Widefield imaging of wild-type HeLa cells cultured for 1h in either: 1) full growth medium (Control); 2) EBSS to starve the cells (Starve); 3) full growth medium plus 100 nM BafA (Control + BafA); or 4) EBSS plus 100 nM BafA (Starve + BafA). Cells were labelled with anti-GALNT2 (Alexa Fluor 488), in combination with either anti-GLG1 (Alexa Fluor 568) or anti-TGOLN2 (Alexa Fluor 680), as shown in Fig. 6, or were labelled with anti-LC3B (Alexa Fluor 488), as shown in Supp. Fig. 6c. In all images, cells were stained with DAPI to label nuclei.

Fig7_GALNT2_GLG1_TM9SF2_TGOLN2_GOLIM4_SDF4: Widefield imaging of HeLa cells left untreated in full growth medium (0h) or cultured in the presence of 100 nM BafA for 0.5, 1, 2, 4, 6 or 8 hours, before fixation. Cells were labelled with anti-GALNT2 (Alexa Fluor 647) in combination with either anti-GLG1, anti-TM9SF2, anti-TGOLN2, anti-GOLIM4 or anti-SDF4 (Alexa Fluor 555). In all images, cells were stained with DAPI and phalloidin-488 to label nuclei and cytoplasm, respectively.