Single-Cell Mapping Reveals Several Immune Subsets Associated with Liver Metastasis of Pancreatic Ductal Adenocarcinoma

Identifying a metastasis-correlated immune cell composition within the tumor microenvironment (TME) of pancreatic ductal adenocarcinoma (PDAC) will help to develop promising and innovative therapeutic strategies. Twenty-six samples from 11 patients (including 11 primary tumor tissues, 10 blood, and 5 lymph nodes) with different stages were used to develop a multiscale immune profile. High-dimensional single-cell analysis with mass cytometry was performed to search for metastasis-correlated immune changes in the microenvironment.

The details about the files uploaded are as follows:

1. panelA_Blood.zip includes 10 .fcs files from blood samples in Panel A;

2. panelA_LN.zip includes 5 .fcs files from lymph node samples in Panel A;

3. panelA_Tumor.zip includes 11 .fcs files from tumor tissue samples in Panel A;

4. panelB_Tumor.zip includes 11 .fcs files from tumor tissue samples in Panel B;

5. panel_metadata.xlsx describes marker used in Panel A and B;

6. sample_metadata.xlsx describes detailed sample information.