Oral chronic toxicity test of nano herbal Phaleria macrocarpa

Extracts of Phaleria macrocarpa have been used for years in traditional medicine and have been evaluated scientifically as well. This study aimed to evaluate the chronic toxicity level of nano herbal Phaleria macrocarpa and its effect on the changes in hematology, biochemistry, electrolytes, and organ histopathology. High Energy Milling (HEM) produced nano herbal Phaleria macrocarpa. By giving nano herbal Phaleria macrocarpa at doses of 300, 600, and 900 mg/kg BW for 56 days, the mice's blood was collected for the hematological and electrolyte parameters. The kidney and liver were assessed for biochemical parameters. As a result, the nano herbal Phaleria macrocarpa affected the histopathological organs, hematological, biochemical, and electrolyte parameters at an appropriate dose of 300–600 mg/kg BW. The maximum period of the administration this herbal medicine is one month. Therefore, with the correct dose and period of administration, this plant can be used as herbal medicine in the future.