SPECTROSCOPIC STUDIES ON THE INTERACTION BETWEEN THE HUMAN HEMOGLOBIN WITH FOOD ADDITIVE AMARANTH NANOPARTICLE USING BINDING SITES AND MOLECULAR DOCKING

The human hemoglobin (HHb) interaction analysis with the Amaranth nanoparticle system (Amt-NS) was investigated using UV-VIS spectrophotometric and spectrofluorimetric techniques, such as synchronous fluorescence, steady state fluorescence, Forster resonance energy transfer (FRET) and time-resolved fluorescence. Amaranth nanoparticle system (Amt-NS) was prepared by ionic gelation method and characterised by particle size analyser, Fourier transform infrared (FTIR) and scanning electron microscope (SEM) analyses. An intimate binding interaction between the Amt-NS (food colourant) and HHb protein was predicted in this study. The findings revealed that Amt-NS altered the environment of tryptophan and tyrosine residues in HHb. The analysis of synchronous fluorescence and time resolved fluorescence confirmed the fluorescence quenching process. There was an estimate of the binding constant and number of binding sites. Molecular docking and antibacterial activity have been studied. The experimental findings of fluorescence were in line with the results obtained from studying molecular docking. The molecular docking experiment revealed the binding mode of the complex of HHb and Amt-NS.