We describe the advertisement call of the Nanling horned frog, Boulenophrys nanlingensis for the first time, based on recordings obtained from four individuals. One of these individuals, which was identified using its dorsum pattern, was recorded twice after nine months. Distinct shifts in the temporal parameters and call rate were observed from calls of the re-captured individual, which was suspected to be related to temperature and social context. However, due to the limited sample size, further research is needed to confirm these findings. We highlight the potential of mark-recapture method using dorsum pattern for studying and monitoring the Nanling horned frog and other megophyinid frogs.