H4K20me3 controls Ash1-mediated H3K36me3 and transcriptional silencing in facultative heterochromatin

Normalized ChIP-seq datasets for visualization in IGV. The tracks contain means of pooled replicate datasets.

ChIP-seq data were quality-filtered and adapters removed with trimmomatic v.0.39 (Bolger et al., 2014). Mapping was performed with bowtie2 v.2.4.4 (Langmead and Salzberg, 2012), and sorting and indexing with samtools v.1.9 (Li, 2011). Normalized coverage bigwig files and heatmaps were created with deeptools v.3.5.1 (Ramírez et al., 2016). Wiggletools v.1.2 and the UCSC Genome Browser tools were used to calculate means for replicates and converting wig to bigwig files.

Reference genome file is modified from Goodwin et al., 2011. Chromosome 18 was removed from the genome as our reference isolate is missing chromosome 18. 

Gene annotation file was obtained from FungiDB (release 53) and is based on the annotation published by Grandaubert et al., 2015.