Accompanying images to the paper NLR immune receptor–nanobody fusions confer plant disease resistance

The following images accompany the paper NLR immune receptor–nanobody fusions confer plant disease resistance, Kourelis J., Marchal C., Posbeyikian A., Harant A., Kamoun S. (2023) Science Vol. 379, No. 6635. DOI: 10.1126/science.abn4116

Figure captions:

Figure 1. Leaves photographed 12 days after infection with a Potato Virus X variant expressing the fluorescent protein EGFP. Left: Nicotiana benthamiana leaf that doesn't express the pikobody system. Right: Leaf from N. benthamiana line stably expressing the Pikobody system recognizing EGFP. 

Figure 2.  Leaves photographed 12 days after infection with a Potato Virus X variant expressing the fluorescent protein mCherry. Both leaves correspond to a N. benthamiana line stably expressing the Pikobody system recognizing EGFP. These results accompany the observations that the Pikobody system targetting EGFP specifically recognizes EGFP, but not mCherry.

Figure 3.  Side by side comparison of representative adult N. benthamiana plants, from left to right: wild-type (WT), stably transformed with the rice NLR pair Pikm-1/2 (Pikm) or Pikobody^Enhancer (Lines #4 - #1 - #9 - #10).