Example of Fluorescence Lifetime Imaging Microscopy (FLIM) image stack in .ptu format

The dataset is a 3D stack of fluorescence lifetime imaging microscopy (FLIM) images in ptu format to be used as test and training data. It contains the original .lif file (1) with the stack and a single plane image (to be opened using LAS X and LAS X SMD FLIM), exported raw FLIM data in .ptu format of the stack (3) and the single plane (2a) (to be opened in software capable of reading .ptu files) as well as an intensity image in .tif format (2b) of the single plane for a quick sample overview.

The sample is a cross-section of hazel (Corylus avellana) 'diclinous male flower t.s.' with Etzold staining provided by the company Zeiss (CZ 01/05). The dataset was generated using a Leica Stellaris 8 upright confocal laser scanning microscope using a 93x/1.4 glycerol immersion objective. Each image of the 65 slice stack with z step size of 0.287 µm contains 512 x 512 pixels with a pixel size of 0.078 µm x 0.078 µm. Excitation was done with a white-light laser at 491 nm and a laser pulse rate of 40 MHz and a pixel dwell time of 2.0875 µs. Images were acquired using a HyD X detector in counting mode in the spectral range of 496 to 739 nm using Leica Application Suite X (LAS X) version 4.4.0.24861 and LAS X SMD FLIM version 4.5.0 for FLIM image acquisition. 10 frames were accumulated per image. Metadata is available as text file (4a) and as metadata files from LAS X (4b).