Published January 26, 2023 | Version 3
Dataset Open

Alternative splicing analysis benchmark with DICAST

Authors/Creators

  • 1. University of Hamburg
  • 2. Technical University of Munich
  • 3. University Medicine Greifswald
  • 4. Technische Universität Braunschweig and Hannover Medical School

Description

Alternative splicing is a major contributor to transcriptome and proteome diversity in health and disease. A plethora of tools have been developed for studying alternative splicing in RNA-seq data. Previous benchmarks focused on isoform quantification and mapping. They neglected event detection tools, which arguably provide the most detailed insights into the alternative splicing process. DICAST offers a modular and extensible framework for analysing alternative splicing, integrating eleven splice-aware mapping and eight event detection tools. We benchmark all tools extensively on simulated as well as whole blood RNA-seq data. STAR and HISAT2 demonstrated the best balance between performance and run time. The performance of event detection tools varies widely, with no tool outperforming all others. DICAST allows researchers to employ a consensus approach to consider the most successful tools jointly for robust event detection. Furthermore, we propose the first reporting standard to unify existing formats and to guide future tool development.

Notes

The archives contain Rscripts used to simulate the data sets; description of AS events and resulting genome annotations in gtf and gff3 formats

Files

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