Published January 26, 2023 | Version v1

Manual time-lapse at 50°C of DNA-droplets after addition of amphiphile

  • 1. Karlsruhe Institute of Technology

Description

Time-lapse microscopy data of DNA-nanomotifs (color channel 1) and amphiphilic nanomotifs (color channel 2), samples were incubated at 50°C in a thermocycler, time-step between measurements is 10 min. Imaging was performed at an ambient temperature of 40°C within the microscope enclosure. The upload contains microscope software data (NIS-elements) and the scripts used for segmentation of droplets and further analysis.

Additional image information

Objective: Apo LWD 40x WI λS DIC N2.

Width:  232.375 microns (1430)
Height:  168.35 microns (1036)
Depth:  12.6 microns (21)
Size:  119MB
Resolution:  6.1538 pixels per micron
Voxel size: 0.1625x0.1625x0.6 micron^3

Files

Analysis_Time_Lapse_Script.ipynb

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