Centro Cardiologico Monzino IRCCS
Published January 16, 2023 | Version v1
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Dataset related to the article "Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A)"

Authors/Creators

  • 1. Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino-IRCCS, Milan, Italy
  • 2. Stem Cell Laboratory, Department of Pathophysiology and Transplantation, Universit`a degli Studi di Milano, Unit of Neurology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Milan, Italy
  • 3. Medical Genetics, Department of Health Sciences, Università degli Studi di Milano, Milan, Italy
  • 4. Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino-IRCCS, Milan, Italy-Department of Biomedical, Surgical and Dental Sciences, Universit`a degli Studi di Milano, Italy

Description

This record contains raw data related to the article " "Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A)"

Abstract

Duchenne muscular dystrophy (DMD) is an X-linked syndrome that affects skeletal and cardiac muscle and is caused by mutation of the dystrophin gene. Induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts by electroporation with episomal vectors containing the reprogramming factors (OCT4, SOX2, LIN28, KLF4, and L-MYC). The donor carried an out-of-frame deletion of exons 45–50 of the dystrophin gene. The established iPSC line exhibited normal morphology, expressed pluripotency markers, had normal karyotype and possessed trilineage differentiation potential.

Notes

Founding: Fondazione IEO-CCM; Italian Ministry of Health-Ricerca Corrente MPP5A: Advanced cell models of cardiomyopathies; Fondazione Umberto Veronesi; Telethon-Unione Italiana Lotta alla Distrofia Muscolare Clinical Projects 2019 GUP19012

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Additional details

Related works

Is supplement to
Journal article: 10.1016/j.scr.2022.102889 (DOI)

Funding

European Commission
ERA-CVD - ERA-NET on cardiovascular diseases to implement joint transnational research projects and set up international cooperations 680969