Published June 7, 2022 | Version v1
Journal article Open

Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase

  • 1. Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747AG Groningen, The Netherlands; Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy
  • 2. Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747AG Groningen, The Netherlands
  • 3. Electronic and Atomic Protein Modelling Group, Barcelona Supercomputing Center, E-08034 Barcelona, Spain
  • 4. Electronic and Atomic Protein Modelling Group, Barcelona Supercomputing Center, E-08034 Barcelona, Spain;
  • 5. Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy
  • 6. Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747AG Groningen, The Netherlands;

Description

Flavin-dependent carbohydrate oxidases are valuable tools in biotechnological applications due to their high selectivity in the oxidation of carbohydrates. In this study, we report the biochemical and structural characterization of recently discovered carbohydrate oxidase from the bacterium Ralstonia solanacearum, which is a member of the vanillyl alcohol oxidase flavoprotein family

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Funding

OXIPRO – Transition towards environment-friendly consumer products by co-creation of an oxidoreductase foundry 101000607
European Commission