Dicrossus foirni sp. n. and Dicrossus warzeli sp. n. are described from the Brazilian Amazon River system. Both species are differentiated from all other Neotropical cichlids by exhibiting three rows of longitudinal lateral spots. Dicrossus foirni sp. n., which is distributed in the northern Rio Negro drainage, exhibits a pattern of lateral spots formed by the clustering of individual dots. By contrast, Dicrossus warzeli sp. n., which is endemic to the Rio Tapajós, exhibits three longitudinal rows of horizontal dash-like spots on the sides of the body. Furthermore, the two species are distinguished by having different relationships between body height and body length.